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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NSF All Species: 30
Human Site: T13 Identified Species: 47.14
UniProt: P46459 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.29
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P46459 NP_006169.2 744 82594 T13 M Q A A R C P T D E L S L T N
Chimpanzee Pan troglodytes XP_511626 842 92301 T86 M Q A A R C P T D E L S L T N
Rhesus Macaque Macaca mulatta XP_001105450 854 93325 T123 M Q A A R C P T D E L S L T N
Dog Lupus familis XP_548044 752 83541 T13 M Q A A R C P T D E L S L T N
Cat Felis silvestris
Mouse Mus musculus P46460 744 82595 T13 M Q A A R C P T D E L S L S N
Rat Rattus norvegicus Q9QUL6 744 82634 T13 M Q A A R C P T D E L S L S N
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus XP_418094 740 82158 T13 M Q A A R C P T D E L S L T N
Frog Xenopus laevis P23787 805 89193 S13 D T K S D D L S T A I L K Q K
Zebra Danio Brachydanio rerio Q7ZU99 806 89405 S13 E S K N D D L S T A I L K Q K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P46461 745 82537 D13 K A T K C P T D E L S L T N R
Honey Bee Apis mellifera XP_001120201 743 82591 D14 K A V R C P T D E L S I T N C
Nematode Worm Caenorhab. elegans Q94392 824 91316 E77 V R K A P S E E H T L A N Y A
Sea Urchin Strong. purpuratus NP_999752 746 82671 D15 K A V R C P T D Q L S L T N R
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q9M0Y8 742 81469 L22 T N T P S A D L A F T N L A Y
Baker's Yeast Sacchar. cerevisiae P18759 758 84038 S32 R T R H L K V S N C P N N S Y
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 88 86.8 98.1 N.A. 98.1 97.8 N.A. N.A. 93.6 26.2 25 N.A. 62.4 65.8 50.6 64.8
Protein Similarity: 100 88.2 87.1 98.8 N.A. 99.7 99.7 N.A. N.A. 96.5 44.2 44.4 N.A. 77.7 81.1 66.2 80.5
P-Site Identity: 100 100 100 100 N.A. 93.3 93.3 N.A. N.A. 100 0 0 N.A. 0 0 13.3 0
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. N.A. 100 20 13.3 N.A. 6.6 6.6 33.3 0
Percent
Protein Identity: N.A. N.A. N.A. 43.5 44.9 N.A.
Protein Similarity: N.A. N.A. N.A. 64.6 64.2 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 0 N.A.
P-Site Similarity: N.A. N.A. N.A. 13.3 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 20 47 54 0 7 0 0 7 14 0 7 0 7 7 % A
% Cys: 0 0 0 0 20 47 0 0 0 7 0 0 0 0 7 % C
% Asp: 7 0 0 0 14 14 7 20 47 0 0 0 0 0 0 % D
% Glu: 7 0 0 0 0 0 7 7 14 47 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 7 0 0 0 0 7 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 14 7 0 0 0 % I
% Lys: 20 0 20 7 0 7 0 0 0 0 0 0 14 0 14 % K
% Leu: 0 0 0 0 7 0 14 7 0 20 54 27 54 0 0 % L
% Met: 47 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 7 0 7 0 0 0 0 7 0 0 14 14 20 47 % N
% Pro: 0 0 0 7 7 20 47 0 0 0 7 0 0 0 0 % P
% Gln: 0 47 0 0 0 0 0 0 7 0 0 0 0 14 0 % Q
% Arg: 7 7 7 14 47 0 0 0 0 0 0 0 0 0 14 % R
% Ser: 0 7 0 7 7 7 0 20 0 0 20 47 0 20 0 % S
% Thr: 7 14 14 0 0 0 20 47 14 7 7 0 20 34 0 % T
% Val: 7 0 14 0 0 0 7 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 7 14 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _